Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells

Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with this easy-to-use, comprehensive kit.

Brand:  Thermo Scientific™ 84840

Product Code. 13454219

Description

Perform preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis with Thermo Scientific™ Pierce Mass Spec Sample Prep Kit for Cultured Cells, an easy-to-use, comprehensive kit.

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The Pierce Mass Spec Sample Prep Kit for Cultured Cells contains all the necessary reagents and enzymes to prepare up to 20 samples (1 million cells each) for MS analysis. The simple and robust workflow uses a lysis protocol that generates approximately 100μg of protein per sample and includes a control digestion indicator to monitor the efficiency of the two-step enzymatic digestion protocol. The kit procedure, which includes a Digestion Indicator as an internal protein/peptide control, produces consistently and reproducibly clean peptide mixtures for protein identification.

Highlights:

• Complete – includes all reagents, a digestion indicator control, proteases and an optimized protocol needed to process up to 20 samples

• Simple – user friendly kit can provide reproducible results even for non-expert MS analysts

• Flexible – can be adapted to handle sample sizes between 10 to 200μg

• High yield – total protein yield from 1 million cells is greater than 100μg

• Optimized – cysteine reduction and alkylation are 100% with less than 1% over alkylation of non-cysteine residues

• Efficient - percentage of missed cleavages is less than 10%

• Compatible – final preparation is ready for direct MS analysis and other downstream applications, including mass-tag labeling

Includes:

Kit contains proprietary lysis and digestion buffers, digestion indicator, DTT, iodoacetamide, Lys-C, trypsin

Specifications

• For Use With (Equipment): Microcentrifuge, Mass Spectrometer
• Product Line: Pierce™
• Starting Material: Mammalian Cells
• Detection Method: Mass Spectrometry
• Quantity: 20 Reaction Kit

• Product Type: Sample Preparation Kit
• Content And Storage: Upon receipt, remove Insert A (containing Pierce Digestion Indicator, Lys-C Protease and Pierce Trypsin Protease, MS Grade) and store at -20°C. Store the remaining components at 4°C. Product is shipped on dry ice.
• Final Product Type: Peptide
• Workflow Step: Protein Digestion, Cell Lysis, Sample Clean Up