Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 31494

MALEIMIDE ACTIVATED HRP, EZ-LINK KIT 00

UNSPSC: 41115823

Code: PN

Additional Details:
Additional Details: Weight: 1.81440kg Transport: UN number : 3335 Chem class : 1 Pack group : III



Product Code. 10454165

Quantity Price
1 £ 296.0 / 1 set
Estimated Shipment date
from Supplier 06-02-2017
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Description and Specification

Specification

Formulation Multi-component kit with activated HRP
Sufficient For 1 enzyme-labeling reaction with up to 5mg of antibody (IgG)
Quantity 1 rxn kit
Includes Maleimide Activated Horseradish Peroxidase, 5mg;;Conjugation Buffer, 20mL;;2-Mercaptoethylamine·HCl, 6mg;;SATA, 2mg;;Dimethylformamide, 1mL;;Hydroxylamine·HCl, 5mg;;Polyacrylamide Desalting Column, 1 x 10mL
Item Description Maleimide Activated Hrp 1/pk

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues