Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit
Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.
Brand: Thermo Scientific™ 31494
Code : PN
Additional Details : Weight : 1.81440kg
DescriptionThe Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.
- Prepare enzyme conjugates from proteins that contain a free sulfhydryl
- Includes 2-MEA to generate free sulfhydryls from disulfide bonds
- Includes SATA to add free sulfhydryls to lysine residues
|Maleimide Activated Horseradish Peroxidase, 5mg;;Conjugation Buffer, 20mL;;2-Mercaptoethylamine·HCl, 6mg;;SATA, 2mg;;Dimethylformamide, 1mL;;Hydroxylamine·HCl, 5mg;;Polyacrylamide Desalting Column, 1 x 10mL|
|Maleimide Activated Hrp 1/pk|
|1 enzyme-labeling reaction with up to 5mg of antibody (IgG)|
|1 rxn kit|
|Multi-component kit with activated HRP|