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Thermo Scientific™ Pierce™ Maleimide-Activated HRP Conjugation Kit

Activated horseradish peroxidase (HRP) enzyme and kit to label proteins, peptides or other ligands via sulfhydryl groups (reduced cysteines) to make HRP conjugates.

Brand:  Thermo Scientific™ 31494

Code : PN

Additional Details : Weight : 1.81440kg Transport : UN number : 3335 Chem class : 1 Pack group : III

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Product Code. 10454165

  • £296.00 / 1 set


Estimated Shipment
06-11-2017
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Description

Description

The Thermo Scientific Maleimide Activated Horseradish Peroxidase (HRP) Kit can be used to directly prepare HRP conjugates from proteins, peptides or other ligands that contain a free sulfhydryl group. This kit eliminates the first step of the two-step maleimide method, making conjugate preparation much simpler. The kit is supplied with a stable, Maleimide-activated horseradish peroxidase. HRP conjugates can be purified by gel filtration chromatography, ultrafiltration or dialysis, depending upon the size of the conjugated protein.

Highlights:

  • Prepare enzyme conjugates from proteins that contain a free sulfhydryl
  • Includes 2-MEA to generate free sulfhydryls from disulfide bonds
  • Includes SATA to add free sulfhydryls to lysine residues
Specifications

Specifications

Maleimide Activated Horseradish Peroxidase, 5mg;;Conjugation Buffer, 20mL;;2-Mercaptoethylamine·HCl, 6mg;;SATA, 2mg;;Dimethylformamide, 1mL;;Hydroxylamine·HCl, 5mg;;Polyacrylamide Desalting Column, 1 x 10mL
Maleimide Activated Hrp 1/pk
1 enzyme-labeling reaction with up to 5mg of antibody (IgG)
1 rxn kit
Multi-component kit with activated HRP