Thermo Scientific™ Pierce™ Iminobiotin and Biotin Resins

Agarose beads of immobilized biotin (and iminobiotin) to strongly (or reversibly) bind and remove (or purify) streptavidin or avidin proteins and conjugates from samples.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 20221

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: CAS Number: 608-28-6 Weight: 0.80000kg



Product Code. 11856734

Quantity Price
1 £ 116.0 / 5mL
Estimated Shipment date
from Supplier 10-04-2017
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Description and Specification

Specification

Formulation Iminobiotin on crosslinked 6% beaded agarose, slurried in water with sodium azide
Sufficient For Binding >/= 1mg of avidin/mL resin
Description Pierce Iminobiotin Agarose
Support Type Agarose
Quantity 5mL

Pierce Biotin Agarose and Iminobiotin Agarose are beaded agarose supports onto which biotin and iminobiotin moieties have been covalently immobilized. Typical avidin-biotin affinity procedures use immobilized avidin or streptavidin proteins to capture biotin-tagged molecules from a sample. Occasionally, however, the reverse situation arises in which immobilized biotin in needed to capture avidin or streptavidin components from a sample. These biotin and iminobiotin resins address this special situation.

Immobilized biotin binds avidin and streptavidin very strongly, making it appropriate for non-reversible binding applications. Immobilized iminobiotin is the guanido analog of biotin. The dissociation constant of the avidin-iminobiotin complex is pH dependent. At pH 9.5 to 11, the avidin-iminobiotin complex will bind tightly. At pH 4, the avidin-iminobiotin complex will dissociate. Thus, iminobiotin agarose can be used for situations requiring reversible binding and elution of avidin components.

Highlights:

  • Enable purification or removal of avidin or streptavidin samples
  • Resins are crosslinked beaded agarose onto which biotin or iminobiotin are covalently immobilized
  • Use Biotin Agarose for non-reversible binding (e.g., removal of avidin components from a sample)
  • Use Iminobiotin Agarose for reversible binding (e.g., non-denaturing purification of avidin)

Documentation

Technical documents