Thermo Scientific™ Pierce™ IgM Purification Kit

Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 21018

IMMUNOPURE MBP COLUMN BUFF 50ML

UNSPSC: 41116126

Code: PN

Additional Details:
Additional Details: CAS Number: 1313-27-5 Weight: 0.80000kg



Product Code. 11896794

Quantity Price
1 £ 38.2 / 50mL
Estimated Shipment date
from Supplier 19-12-2016
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Description and Specification

Specification

Description Pierce MBP Column Preparation Buffer
Formulation Tris buffer with sodium chloride, sodium azide and EDTA; pH 7.4
Quantity 50mL
Sufficient For Preparing mannan binding protein column for binding mouse IgM

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.

Highlights:

  • Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum

  • Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use

  • Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies

  • Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity

Includes:

Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender