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Thermo Scientific™ Pierce™ IgM Purification Kit

Easily purify IgM from mouse ascites fluid using immobilized mannan binding protein, which binds to IgM in the kit-supplied buffers.

Brand:  Thermo Scientific™ 22212

UNSPSC : 41116126

Code : PN

Additional Details : CAS Number : 7493-95-0 Weight : 0.80000kg

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Product Code. 11821275

  • £964.00 / 10mL


Estimated Shipment
12-07-2017
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Specifications

Description

Pierce MBP Agarose is purified mannan binding protein (also called mannose-binding lectin, MBL) that has been immobilized on 4% beaded agarose. When used with an optimized buffer system based on temperature- and calcium-dependent binding, this resin enables specific affinity purification of mouse IgM from ascites fluid and other concentrated samples. The resin and essential buffers are available individually or bundled together in a complete IgM Purification Kit containing a pre-packed 5mL column of the MBP Agarose.

Highlights:

  • Immobilized MBP – affinity system based on mannan binding protein (MBP) allows specific purification of mouse IgM from ascites fluid or partial isolation of human IgM from serum

  • Complete kit – IgM Purification Kit includes a pre-packed MBP column and ready-to-use buffers for convenient purification of at least 1.5mg IgM from 0.5mL ascites samples per column-use

  • Mild conditions – nondenaturing, near-neutral buffer conditions ensure recovery of functional antibodies

  • Reusable – stable ligand and resins can be regenerated and reused at least 10 times without significant decline in binding capacity

Includes:

Kit contains a 5mL MBP Agarose Column, column preparation buffer, IgM binding and elution buffers, and a column extender

Description & Specifications

Specifications

Pierce Mannan Binding Protein Agarose
10mL
Resin slurry
IgM (mouse)
Mannan binding protein (MBP) on 4% beaded agarose, slurried in IgM Binding Buffer
Binding 0.3mg mouse IgM from 0.1mL ascites fluid per 1mL resin per use
Agarose