Thermo Scientific™ Pierce™ High Capacity Endotoxin Removal Spin Columns, 1 mL

Product Code.	Quantity	unitSize
12391973	5 Columns	Pack of 5
12201203	25 Columns	Pack of 25

Product Code. 12391973 Supplier Thermo Scientific™ Supplier No. 88276

Description

Remove endotoxins (lipopolysaccharides or LPS) from samples before mass spectrometry using spin columns of poly(e-lysine) ligand on beaded cellulose.

Thermo Scientific Pierce High Capacity Endotoxin Removal Resin binds to reduce endotoxin levels in protein samples by ≥99% in 1 hour; with this spin cup format, protein recovery is ≥85%.

Features of High Capacity Endotoxin Removal Resin Spin Columns:

• High capacity—bind up to 2,000,000 EU/mL^† to eliminate >99% of endotoxins
• Selective—recover ≥85% of your protein sample
• High performance—meets FDA guidelines by reducing final EU concentration to <5 EU/mL
• Fast—our new spin cup format enables endotoxin depletion within 1 hour
• Clean—single-use spin columns avoid cross contamination of samples
• Optimized—spin cups are optimized for different sample volumes

The Pierce High Capacity Endotoxin Removal Resin combines porous cellulose beads and an FDA-approved food preservative, poly(ε-lysine), as an affinity ligand to selectively bind endotoxins. The modified polylysine affinity ligand eliminates the toxicity concerns associated with alternative technologies using polymixin B ligands and sodium deoxycholate buffers.

Requires:

• Endotoxin-free water

Applications:

• Eliminate endotoxins from protein or antibody samples prior to cell transfection or animal injection

The presence of endotoxins in biologically derived products prepared for research, vaccination, or therapeutic use is a major concern due to the diverse and potentially harmful biological response to these molecules. Endotoxin contamination in proteins purified from bacterial cultures is due to residual lipopolysaccharides (LPS) derived from the outer cell membrane of gram-negative bacteria such as E. coli. Endotoxin-contaminated protein samples transfected into cells or injected into an animal host can initiate a strong immune response resulting in a Systemic Inflammatory Response Syndrome (SIRS) and/or sepsis. Traditional endotoxin removal resins that use polymixin B and sodium deoxycholate to clear endotoxins from a sample also have the negative side effects of neurotoxicity and acute renal tubular necrosis.

Pierce High Capacity Endotoxin Removal Resin is a porous cellulose bead that has been surface-modified with covalently attached polylysine chains that have a high affinity for LPS but lacks the toxicity of other endotoxin-binding molecules. With a 2,000,000-EU/mL binding capacity, endotoxin levels can be reduced by 99% in samples containing initial endotoxin levels of 10,000 EU/mL. Typical protein samples processed with the High Capacity Endotoxin Removal Resin have a final endotoxin concentration below 5 EU/mL. This spin cup format is a fast, single-use method to remove endotoxins from protein samples in as little as 1 hour. The spin cups use a batch format to bind and remove endotoxins while allowing for >85% protein recovery.

^† One endotoxin unit/mL (EU/mL) equals approximately 0.1ng endotoxin/mL of solution.

Related Products

• Pierce™ High Capacity Endotoxin Removal Resin (Cat. No. 88270)
• Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.25 mL (Cat. No. 88273)
• Pierce™ High Capacity Endotoxin Removal Spin Columns, 0.5 mL (Cat. No. 88274)

Specifications

• Capacity (Metric): Up to 2,000,000 EU/mL
• Formulation: 22 mL-capacity centrifuge columns containing 1 mL resin
• Content And Storage: Upon receipt store at 4°C.
• Form: Liquid
• Product Line: Pierce
• Quantity: 5 Columns
• Target: Endotoxin Removal
• Type: Spin Column
• Stationary Phase: Proprietary Ligand
• Format: Spin Column
• Column Type: Affinity, Cellulose Resin

Safety and Handling

Product Identifier

• Pierce High Capacity Endotoxin Removal Spin Columns, 1 mL

Signal Word

• Danger

Hazard Category

• Carcinogenicity Category 2
• Serious eye damage/eye irritation Category 2
• Flammable liquid Category 3
• Reproductive toxicity Category 1
• Skin corrosion/irritation Category 2

Hazard Statement

• H226-Flammable liquid and vapour.
• H315-Causes skin irritation.
• H319-Causes serious eye irritation.
• H340-May cause genetic defects.
• H351-Suspected of causing cancer.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P302+P352-IF ON SKIN: Wash with plenty of water/soap.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P405-Store locked up.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contain: ethanol

For Research Use Only. Not for use in diagnostic procedures.