Thermo Scientific™ Pierce™ GST Agarose

Isolate or remove cross-reacting GST-specific serum or other antibody solutions with immobilized glutathione S-transferase agarose.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 20211

PIERCE AGAROSE GST 5ML

UNSPSC: 41106510

Code: PN

Additional Details:
Additional Details: CAS Number: 1499-33-8 Weight: 0.80000kg



Product Code. 11816734

Quantity Price
1 £ 426.0 / 5mL
Estimated Shipment date
from Supplier 23-12-2016
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Description and Specification

Specification

Description Pierce GST Agarose
Formulation Glutathione S-transferase on crosslinked 6% beaded agarose, slurried in water with sodium azide
Quantity 5mL
Sufficient For Binding approx. 0.5mg anti-GST antibody per mL of resin
Support Type Agarose

This GST resin consists of purified glutatione S-transferase protein from Schistosoma japonicum that has been immobilized on crosslinked 6% beaded agarose. Antibody molecules in that cross-react with the GST portion of a recombinant fusion protein can be removed from the desired protein-specific antibody by passing antiserum over a GST agarose column. GST antibodies will bind to the immobilized GST, whereas antibodies against other fusion proteins will pass through the column unrestrained.

Highlights:

  • Specific – provides purification of target antibodies from those directed against the GST tag

  • Reusable – bound antibodies can be eluted with 6M guanidinium chloride allowing for 10 purifications with each column or aliquot of resin

  • Two formats – choose either pre-packed columns or resin slurry for use at a variety of scales