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Thermo Scientific™ Pierce™ Glycoprotein Staining Kit

Rapidly stain glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method in this convenient kit.

Brand:  Thermo Scientific™ 24562

235.34 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : PN

Additional Details : Weight : 2.26800kg Transport : UN number : 3316 Chem class : 9 Pack group : II


Product Code. 10688864

  • £353.00 / 1 set
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Description

Description

Thermo Scientific™ Pierce Glycoprotein Staining Kit is a convenient, fast and sensitive kit for staining glycosylated proteins in polyacrylamide gels using the periodic acid-Schiff (PAS) method.

The kit provides three essential reagents and a complete protocol to specifically stain glycosylated proteins (glycoproteins) that have been separated by polyacrylamide gel electrophoresis (PAGE). A gel or membrane containing separated proteins is treated with a periodate solution, which oxidizes cis-diol sugar groups in glycoproteins. The resulting aldehyde groups are detected through the formation of Schiff-base bonds with a reagent that produces magenta bands. Traditionally called the periodic acid-Schiff (PAS) reagent method, this staining technique is made easier by the convenient packaging of essential reagents in one kit with easy-to-follow instructions. The kit includes positive and negative control proteins.

Highlights:

  • Specific for glycoproteins – periodic acid-Schiff (PAS) method specifically detects glycosylated proteins having sialic acid and other oxidizable carbohydrate groups

  • Optimized protocol – the traditional three-reagent method has been streamlined to achieve optimal staining in less than two hours (half the time required for other staining methods)

  • Colorimetric – glycoproteins are detected as magenta bands against a faint pink or colorless gel background

  • Qualitative staining – detection limit depends on the extent of glycosylation and protein size and abundance; detects 0.625ng avidin and 0.16g horseradish peroxidase per band

  • Controls included – complete kit includes positive and negative control proteins (HRP and STI, respectively).

  • Gels and membranes – effective for use with polyacrylamide mini gels or nitrocellulose membranes

Includes:

Three reagents in 250mL bottles (1 solution and 2 dry powders to which water must be added), plus two vials of lyophilized proteins (1mg each)

Specifications

Specifications

Sufficient For: 10 mini gels (SDS-PAGE)
• Oxidation Reagent, 2.5 g powder to make 250 mL
• Reduction Reagent, 1.25 g powder to make 250 mL
• Positive Control (Horseradish Peroxidase), 1 mg
• Negative Control (Soybean Trypsin Inhibitor), 1 mg
• Glycoprotein Stain, 250 mL

Store all components at 4°C.
Colorimetric
Glycoprotein Staining Kit
Proteins (Glycoproteins)
In-Blot Detection, In-Gel Detection
Fuchsin Sulfite
Pierce™
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Safety and Handling

Safety and Handling

Product Identifier
  • Pierce Glycoprotein Staining Kit

Signal Word
  • Danger

Hazard Category
  • Oxidising solid Category 2
  • Acute toxicity Category 4
  • Skin corrosion/irritation Category 1 B
  • Serious eye damage/eye irritation Category 1
  • Skin sensitiser Category 1
  • Respiratory sensitiser Category 1
  • Carcinogenicity Category 1B

Hazard Statement
  • H272-May intensify fire; oxidiser.
  • H302-Harmful if swallowed.
  • H314-Causes severe skin burns and eye damage.
  • H317-May cause an allergic skin reaction.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H350-May cause cancer.
  • EUH031-Contact with acids liberates toxic gas.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-¢In case of inadequate ventilation! wear respiratory protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P220-Keep/Store away from clothing/combustible materials.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P264-Wash thoroughly after handling.
  • P270-Do not eat, drink or smoke when using this product.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
  • P331-Do NOT induce vomiting.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305-IF IN EYES:.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P501-Dispose of contents/container to .

SDS
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Special Offers

Special Offers

For Research Use Only. Not for use in diagnostic procedures.