Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit

Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 23259

LYOPHILIZED GLYCOPROT ST&ARDS SET

UNSPSC: 41116012

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg



Product Code. 10760985

Quantity Price
1 £ 202.0 / 1 set
Estimated Shipment date
from Supplier 14-12-2016
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  • Description and Specification
  • Documentation
  • Accessories and Spare Parts
Description and Specification

Specification

Description Pierce Glycoprotein Standards Set
Formulation Multi-component kit
Kit Contents Apo-tranferrin, lyophilized, 2.5mg
Quantity 5 protein kit
Sufficient For Preparing standard curves for glycoprotein carbohydrate estimation

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

  • Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars

  • Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards

  • Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols

  • Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Includes:

Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards

Recommended for:

Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Documentation

Technical documents

Accessories and Spare Parts