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Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit

Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).

Brand:  Thermo Scientific™ 23260

UNSPSC : 41116012

Code : PN

Additional Details : Weight : 0.10000kg

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Product Code. 10677914

  • £329.00 / 1 set


EU Stock 1
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Description

Description

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Highlights:

  • Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars

  • Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards

  • Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols

  • Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Includes:

Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards

Recommended for:

Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Specifications

Specifications

Glycoprotein Assay Buffer, 250mL
Multi-component kit
60 tube assays or 250 microplate assays
Pierce Glycoprotein Carbohydrate Estimation Kit
500mg kit
Documentation

Technical Documents