Thermo Scientific™ Pierce™ Glycoprotein Carbohydrate Estimation Kit
Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).
Brand: Thermo Scientific™ 23260
Code : PN
Additional Details : Weight : 0.10000kg
DescriptionThe Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (% carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.
Qualitative – easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
Semi-quantitative – estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
Simple procedure – completed in less than 75 minutes; instructions include microplate and test tube protocols
Adaptable – standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components
Sodium periodate, assay buffer, detection reagent and a 5-vial set of glycoprotein standards
Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents; Characterize and classify purified protein fractions from an affinity procedure; Assess sugar and carbohydrate contamination in non-glycosylated protein samples
|Glycoprotein Assay Buffer, 250mL|
|60 tube assays or 250 microplate assays|
|Pierce Glycoprotein Carbohydrate Estimation Kit|