Thermo Scientific™ Pierce™ Fast Wash Buffer, 10X

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with this ready-to-use blocker solution.

Brand:  Thermo Scientific™ 37577

Product Code. 13404299

Description

Optimize Western blotting systems by decreasing both background signal and incubation time compared to traditional blocking agents with Thermo Scientific™ Pierce Fast Blocking Buffer, a ready-to-use blocker solution.

Additional Information and Online Ordering »

Pierce Fast Blocking Buffer streamlines Western blot protocols by providing consistent, high-quality blocking for nitrocellulose or PVDF membranes in only 5 minutes. Blocking and probing steps with Fast Blocking Buffer prevents nonspecific binding of detection antibodies during probing steps but allows specific detection to occur. Pierce Fast Wash Buffer provides efficient membrane washing after incubation with a primary and secondary antibody to effectively shorten total washing time to 20 minutes (from the typical 90 minutes). These two buffers are compatible with antibodies and biotin-avidin systems, as well as both nitrocellulose and PVDF membranes. The blocking buffer provides rapid and effective blocking in just five minutes while producing results comparable to traditional blockers for Western blots.

Highlights:

• Fast – use both buffers to shorten the typical Western blot development by over 2 hours

• Simple – optimized protocol makes Western blot analysis easier than ever

• Low background – provides results comparable to classic Western blotting buffers

Specifications

• Description: Pierce Fast Wash Buffer, 10X
• Quantity: 1 L
• For Use With (Application): Western Blot
• Content And Storage: Upon receipt store at 4°C.

• Product Type: Wash Buffer
• Form: Liquid
• Product Line: Pierce™

Safety and Handling

Product Identifier

• Pierce Fast Wash Buffer, 10X

Signal Word

• Warning

Hazard Category

• Serious eye damage/eye irritation Category 2

Hazard Statement

• H319-Causes serious eye irritation.

Precautionary Statement

• P264-Wash hands thoroughly after handling.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.

Supplemental information

• MIXTURE LIST-Contains : sodium chloride, trometamol, Poly(oxy-1,2-ethanediyl),,1,3,3-tetramethylbutyl)phenyl]-

For Research Use Only. Not for use in diagnostic procedures.