Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate
Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
Brand: Thermo Scientific™ 32134
Code : PN
Additional Details : CAS Number : 123-91-1 Weight : 0.25000kg Transport : UN number : 1993 Chem class : 3 Pack group : II
DescriptionPierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.
Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
Higher sensitivity — detect targets down to the low-picogram level
Longer signal duration — sustained light output for as long as five hours
More imaging options — X-ray, CCD or laser-based imagers
More affordable — high quality and performance at a lower price than other suppliers« similar substrates
|Detection Reagent A, 25mL, Detection Reagent B, 0.625mL|
|250cm2 of membrane|
|Pierce ECL Plus Western Blotting Substrate|
|Proprietary acridan and peroxide solutions|
|2° to 8°C|