Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Brand: Thermo Scientific™

Manufacturer Part Number: 32134

ECL Plus Western Blotting Substrate, 25ml SAMPLE

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 123-91-1 Weight: 0.25000kg Transport: UN number : 1993 Chem class : 1 Pack group : II

Product Code. 11547910

Quantity Price
1 £87.00 / 25mL
EU Stock 4
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Description and Specification


Conjugate HRP
Description Pierce ECL Plus Western Blotting Substrate
Detection Chemiluminescent
Formulation Proprietary acridan and peroxide solutions
Kit Contents Detection Reagent A, 25mL
Quantity 25mL kit
Sufficient For 250cm2 of membrane
Storage Requirements 2° to 8°C

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.


  • Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

  • Higher sensitivity — detect targets down to the low-picogram level

  • Longer signal duration — sustained light output for as long as five hours

  • More imaging options — X-ray, CCD or laser-based imagers

  • More affordable — high quality and performance at a lower price than other suppliers« similar substrates