Thermo Scientific™ Pierce™ ECL Plus Western Blotting Substrate

Acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 32134

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 123-91-1 Weight: 0.25000kg Transport: UN number : 1993 Chem class : 1 Pack group : II



Product Code. 11547910

Quantity Price
1 £ 95.5 / 25mL
EU Stock 5
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Description and Specification

Specification

Formulation Proprietary acridan and peroxide solutions
Storage Requirements 2° to 8°C
Kit Contents Detection Reagent A, 25mL
Conjugate HRP
Sufficient For 250cm2 of membrane
Description Pierce ECL Plus Western Blotting Substrate
Detection Chemiluminescent
Quantity 25mL kit

Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for Western blotting.

Highlights:

  • Easy to use — can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization

  • Higher sensitivity — detect targets down to the low-picogram level

  • Longer signal duration — sustained light output for as long as five hours

  • More imaging options — X-ray, CCD or laser-based imagers

  • More affordable — high quality and performance at a lower price than other suppliers« similar substrates