Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns and Filter Plates, 0.5–10 mL

Product Code.	Quantity	Format	Volume (Metric)	Unit Size
17176368	2 x 25 Columns	Spin Column	0.5 mL	Pack of 50
17175762	5 Columns	Spin Column	0.5 mL	Pack of 5
17165762	25 Columns	Spin Column	0.5 mL	Pack of 25
17185762	5 Columns	Spin Column	2 mL	Pack of 5
16627182	25 Columns	Spin Column	2 mL	Each
17195762	5 Columns	Spin Column	5 mL	Pack of 5
16685344	25 Columns	Spin Column	5 mL	Each
16607425	5 Columns	Spin Column	10 mL	Each
17186368	5 x 5 Columns	Spin Column	10 mL	Pack of 25
17105772	2 Plates	96-well Filter Plate	96-well	Pack of 2
17196368	2 x 2 Plates	96-well Filter Plate	96-well	Pack of 4

Artikelnummer. 17176368 Lieferant Thermo Scientific™ Lieferanten-Nr. A44297

Beschreibung

Enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Quickly remove non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples with a 7K MWCO in fewer than 15 minutes with Thermo Scientific Pierce Zeba Dye and Biotin Removal columns and filter plates.

Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. Pierce Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. When using the appropriate amount of sample and working within buffer conditions, these columns and filter plates can successfully remove almost any fluorescent dye. Even dilute protein samples (25 μg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). These products are not recommended for desalting or buffer exchange.

Features of Zeba Dye and Biotin Removal columns and filter plates include:
• Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions
• High recovery—low-binding resin maximizes protein recovery
• Easy-to-use—no cumbersome column preparation or equilibration
• Fast—small-molecule removal and protein recovery in less than 15 minutes
• Flexible—available in spin columns and filter spin plates for a range of needs

Sample capacities
• 0.5-mL spin column: 50 to 120 μL sample
• 2-mL spin column: 400 to 700 μL sample
• 5-mL spin column: 1 to 2 mL sample
• 96-well filter plate: 40 to 100 μL sample (per well)

Available formats
• Spin columns: 0.5, 2, 5, and 10 mL
• 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups

Applications
• Ideal for post-reaction clean-up of samples
• To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery

Spezifikation

• Content And Storage: Store in refrigerator (2–8°C).
• Shipping Condition: Room Temperature
• Format: Spin Column
• Column Type: Proprietary Resin
• Product Line: Zeba
• Quantity: 2 x 25 Columns
• Volume (Metric): 0.5 mL
• Sample Volume (Metric): 50 to 120 μL

Frequently Asked Questions (FAQs)

Are Zeba Dye and Biotin Removal Spin Columns/Filter Plates compatible with mass spectrometry?

Zeba Dye and Biotin Removal Spin Columns/Filter Plates are not directly compatible with mass spectrometry.

Have you tested the Zeba Dye and Biotin Removal Spin Columns/Filter Plates using fixed angle rotor and swinging bucket rotor centrifuges?

The performance of the Zeba Dye and Biotin Removal resin is not affected by the type of centrifuge rotor.

How dilute can my protein sample be for using with Zeba Dye and Biotin Removal Spin Columns/Filter Plates?

Protein samples with as low a concentration as 25 µg/mL can be successfully processed with Zeba Dye and Biotin Removal Spin Columns/Filter Plates to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa).

When should I use Zeba Dye and Biotin Removal Spin Columns/Filter Plates?

Zeba Dye and Biotin Removal Spin Columns/Filter Plates enable fast and efficient removal of non-conjugated fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. We have tested removal of the following:
- Alexa Fluor dyes: 488, 555, 647
- Dylight dyes: 488, 550, 650, 650-4x PEG
- Rhodamine dye
- Biotin
- Reducing agents: DTT, TCEP
- Crosslinker: BS3