Thermo Scientific™ Pierce™ DAB Substrate Kit

Chromogenic horseradish peroxidase (HRP) substrate for Western blot and tissue staining methods.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 34001

# DAB, 10 gm

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 2935-95-2 Weight: 0.01000kg



Product Code. 13232823

Quantity Price
1 £ 99.0 / 10g
Estimated Shipment date
from Supplier 19-12-2016
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Description and Specification

Specification

Conjugate HRP
Description Pierce DAB Powder
Detection Chromogenic
Formulation 3,3'-Diaminobenzidine tetrahydrochloride powder (>/= 97.5% pure)
Kit Contents Single component
Quantity 10g
Sufficient For 2000 uses at 1X (0.5mg/mL) and 10mL each

DAB (3,3'-diaminobenzidine tetrahydrochloride; MW = 214.1) reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples. The brown precipitate is insoluble in alcohol and other organic solvents, making it an excellent substrate for immunohistochemical staining that requires the use of traditional counterstains and mounting media. The Pierce DAB Substrate Kit includes a 10X solution of DAB and Stable Peroxide Substrate Buffer. Crystalline DAB (powder) is also available.

Highlights:

  • HRP substrate – for detection of horseradish peroxidase activity on solid media, including nitrocellulose and PVDF membranes and fixed tissue samples

  • Chromogenic – no special equipment needed for visualization; the DAB reaction produces brown bands or spots at sites of reaction with HRP-conjugated antibodies or probes

  • Package options – choose powder for custom formulation with buffer and peroxide or a complete kit of preformulated solutions for immediate use

Documentation

Technical documents