Thermo Scientific™ Pierce™ Cypridina Luciferase Glow Assay Kit

Product Code. 12201622

Brand: Thermo Scientific™ 16170

Description

Obtain bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

The Thermo Scientific™ Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

Highlights:

• Sensitive – highly sensitive detection of Cypridina luciferase activity

• Secreted luciferase – allows real-time assays and kinetic studies without destroying cells

• Stable – reagents provide increased signal stability compared to flash assays

• Simple – does not require a luminometer with injectors

• Compatible – assay reagents compatible with other Cypridina or Vargula luciferases

• Automation-friendly – amenable to high throughput assays

• Convenient – contains a universal cell lysis buffer and optimized glow assay reagent

• Safe – allows one to perform non-radioactive assays

Includes:

Cell lysis buffer, reaction buffer and substrate

Requires:

Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers

Recommended for:

Promoter studies for analyzing cis- regulatory elements and trans-acting factors; Drug screening; siRNA and miRNA screening; Multiplexed assays to study off-target effects; Secretory pathway / protein localization reporter assays; Signal transduction pathway analysis; RNA splicing studies

Specifications

• Type: Assay Kit
• Target: Luciferase, Cypridina Luciferase
• Quantity: 100 Reaction Kit
• Content And Storage: • Cypridina Glow Assay Buffer, 5 mL, store at 4°C
  • Vargulin (1X), 5 μL, store at -20°C
  • 2X Cell Lysis Buffer, 6 mL, store at room temperature
  
  Upon receipt store kit at -20°C or store individual components as indicated above.
• Assay: Reporter Enzyme, Luciferase Reporter Assay
• Compatible Cells: Mammalian Cells
• Detection Method: Bioluminescence
• For Use With (Equipment): Luminometer (Microplate)
• Format: 384-well plate, 96-well plate
• Label Type: Enzyme Labeled
• Substrate Properties: Chemical Substrate
• Technique: Enhanced Chemiluminescence
• Substrate Type: Luciferase Substrate
• Product Line: Pierce
• Substrate: Vargulin (Cypridina Luciferin)

Safety and Handling

Product Identifier

• Pierce Cypridina Luciferase Glow Assay Kit

Signal Word

• Danger

Hazard Category

• Acute toxicity Category 3
• Serious eye damage/eye irritation Category 1
• Flammable liquid Category 2
• Skin corrosion/irritation Category 1 A
• Specific target organ toxicity after single exposure Category 1

Hazard Statement

• H225-Highly flammable liquid and vapour.
• H301-Toxic if swallowed.
• H311-Toxic in contact with skin.
• H314-Causes severe skin burns and eye damage.
• H331-Toxic if inhaled.
• H370-Causes damage to organs.

Precautionary Statement

• P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
• P233-Keep container tightly closed.
• P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
• P243-Take precautionary measures against static discharge.
• P260-Do not breathe dust/fume/gas/mist/vapours/spray.
• P264-Wash hands thoroughly after handling.
• P270-Do not eat, drink or smoke when using this product.
• P271-Use only outdoors or in a well-ventilated area.
• P280-Wear protective gloves/protective clothing/eye protection/face protection.
• P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor /
• P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
• P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
• P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
• P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
• P308+P313-IF exposed or concerned: Get medical advice/attention.
• P363-Wash contaminated clothing before reuse.
• P403+P235-Store in a well-ventilated place. Keep cool.
• P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.

Supplemental information

• MIXTURE LIST-Contains : methanol

For Research Use Only. Not for use in diagnostic procedures.