The Compat-Able Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 minutes with just a few simple steps. The Compat-Able Preparation Set is available individually or conveniently bundled with either the Thermo Scientific Pierce BCA Protein Assay Kit or the Thermo Scientific Pierce Coomassie Plus Protein Assay Kit.
Eliminate incompatibilities – removes nearly any substance that interferes with colorimetric protein assays: salts, detergents, denaturants, reducing agents, and even IEF sample buffer
Simple and fast – prepare samples in less than 10 minutes with four simple steps
Convenient – reagent set is easier to use than homemade TCA or acetone precipitation reagents, thereby producing more consistent results
Scalable – microcentrifuge method is easily scaled to larger centrifuge tube volumes for special needs
Validated – tested and offered together with BCA and Coomassie Plus Protein Assay Kits; protocol integrates control and normalization procedures
Set contains two bottled reagents (Reagent 1 and Reagent 2); Complete kits contain the Reagent Set plus components of the respective protein assay