Invitrogen™ PARP (Cleaved) Multispecies In-Cell ELISA Kit, Near Infrared

In-cell ELISA Kit

Brand:  Invitrogen™ 62224

Product Code. 11577950

Description

The Cleaved PARP Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells. Principle of the method This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

Poly ADP-Ribose Polymerase (PARP) uses nicotinamide adenine dinucleotide (oxidized form) NAD as a substrate to catalyse the transfer of ADP-ribose to a variety of nuclear protein acceptors. Proteolysis of PARP to its stable 85kDa fragment is an early marker of programmed cell death (apoptosis) and is mediated by the caspase CPP32 protein. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species.

Specifications

• Accession Number: P09874
• Conjugate: Unconjugated
• Product Type: ELISA Kit
• Sufficient For: 96 microplate wells of overnight-cultured cells
• For Use With (Equipment): Fluorescent Microplate Reader
• Gene Symbol: ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1
• Label or Dye: DyLight™ 680, DyLight™ 800
• Regulatory Status: RUO
• Storage Requirements: 2°C to 8°C
• Test Time: 1 hr. 20 min.

• Description: Pierce Cleaved PARP Near Infrared In-Cell ELISA Kit
• Immunoassay Kit Format: Multi-component kit with fluorescent conjugates
• Sample Type: Supernatant
• Target Species: Human
• Gene ID (Entrez): 142
• Kit Contents: Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, DyLight™ 680 Goat Anti-Mouse IgG (H+L), DyLight 800 Goat Anti-Rabbit IgG (H+L), Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2
• Quantity: 96 Tests
• Sample Volume: Supernatant, 10,000 cells
• Target: Human
• Total Assay Time: 24 hr.