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Invitrogen™ Caspase 3 (Cleaved) Multispecies In-Cell ELISA Kit, Near Infrared

In-cell ELISA Kit

Brand:  Invitrogen™ 62223

Code : PN

Additional Details : Weight : 0.01000kg

Product Code. 11568110

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Description

Description

The Cleaved Caspase 3 Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells. Principle of the method This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits,large and small,that dimerize to form the active enzyme. This protein cleaves and activates caspases 6,7 and 9,and the protein itself is processed by caspases 8,9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein,which is associated with neuronal death in Alzheimer′s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
Specifications

Specifications

P42574
Unconjugated
ELISA Kit
96 microplate wells of overnight-cultured cells
Fluorescent Microplate Reader
CPP32,CPP32B,SCA-1
DyLight™ 680, DyLight™ 800
RUO
2°C to 8°C
1 hr. 20 min.
Pierce Cleaved Caspase 3 Near Infrared In-Cell ELISA Kit
Multi-component kit with fluorescent conjugates
Supernatant
Human
836
Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, DyLight™ 680 Goat Anti-Mouse IgG (H+L), DyLight 800 Goat Anti-Rabbit IgG (H+L), Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2
96 Tests
Supernatant, 10,000 cells
Human
24 hr.
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