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Thermo Scientific™ Pierce™ Chemiluminescent Transcription Factor Assay Kits

Capture and measure NF-kappa B (NFkB) p50 or p65 transcription factors in 96-well plates using using ELISA-like plate assays and a SuperSignal ECL substrate.

Brand:  Thermo Scientific™ 89858

Code : PN

Additional Details : Weight : 3.62870kg

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Product Code. 10541384

This item has been discontinued and is no longer available. View the product for possible alternatives or contact our Product Support team on 01509 555888 for assistance



These NF kappa B p50 and p65 Transcription Factor Assay Kits are complete systems containing all the necessary components to measure activated transcription factors in a 96-well microplate format. Kits contain two streptavidin-coated 96-well plates with the bound biotinylated-consensus sequence for the respective factor. The consensus duplexes bind only the active forms of the target transcription factors, diminishing nonspecific binding. This provides greater signal-to-noise ratios than a traditional ELISA. The captured active transcription factor is then detected with specific primary antibody (NFkB p50 or NFkB p65) and a secondary HRP conjugated antibody. Finally, a chemiluminescent substrate is then added to the wells, allowing quantitative measurement with a luminometer or CCD camera.


  • Sensitivity – chemiluminescent substrate yields results 20 to 140 times more sensitive than the colorimetric methods with a broader dynamic range

  • Speed – format allows researcher to perform multiple tests in three to four hours

  • Reliability – each kit contains a positive control lysate, as well as both wild-type and mutant competitor duplexes, ensuring reliable results with a gauge for specificity

  • High-throughput compatibility – 96-well format increases sample throughput over traditional EMSA

  • Convenience – kits are complete and contain all the reagents for two 96-well assay plates

  • Versatility – chemiluminescent signal is detectable using a luminometer or CCD Camera


Two 96-well plates; 5X binding buffer; 20X poly dI-dC; Primary antibody; Antibody dilution buffer; HRP-conjugated secondary antibody; Luminol/enhancer solution; Stable peroxide solution; Two plate sealers; 10X wash buffer; Wild type and mutant duplex; Activated HeLa cell nuclear extract (positive control)



Pierce NF-kappaB p50 Transcription Factor Assay Kit
Luminol/Enhancer Solution, 12mL, NFBkappa p50 Primary Antibody, 24uL, Poly dI-dC (20X), 700uL, Stable Peroxide Solution, 12mL, Binding Buffer (5X), 1 x 1.3mL, Antibody Dilution Buffer, 60mL, 96-Well Assay Plates, 2 plates, HRP-conjugated Secondary Antibody, 20uL
192 assays with 10 to 25uL nuclear or whole cell protein extract
Multi-component kit
2 x 96 test kit