Thermo Scientific™ CHAPS Detergent (3-((3-cholamidopropyl) dimethylammonio)-1-propanesulfonate)

Product Code.	Quantity	unitSize
11851445	5 g	5g
11841445	100 g	100g

Product Code. 11851445 Supplier Thermo Scientific™ Supplier No. 28300

Description

Non-denaturing Zwitterionic detergents that disrupt nonspecific protein interactions with less protein aggregation than nonionic detergents.

Thermo Scientific™ CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

CHAPS is a sulfobetaine derivatives of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and are easily removed from solution by dialysis because of their high critical micelle concentration (CMC).

Highlights:

• Versatile zwitterionic detergents
• Provide mild but effective lysis of cultured mammalian cells
• Nondenaturing and generally do not inactivate protein functions
• Can be removed from solution by dialysis
• Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Properties of CHAPS detergent:

• Chemical Name: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
• Molecular Weight: 614.88
• Detergent Class: Zwitterionic
• Aggregation Number: 10
• Micelle Molecular Weight: 6149
• Critical Micelle Concentration (CMC): 8 to 10mM (0.4920 to 0.6150%, w/v)
• Cloud Point: ≥ 100°C
• Dialyzable: Yes

 

Specifications

• Product Type: Detergent
• Content And Storage: Store at Room Temperature
• Form: Powder
• Reagent Type: Detergent (Pure)
• Quantity: 5 g

Frequently Asked Questions (FAQs)

Are there mass spectrometry reagents for membrane proteins?

CHAPS (Cat. Nos. 28299, 28300), OTG (Cat. No. 28351) and Sodium Deoxycholate (Cat. Nos. 89904, 89905) are detergents often used to solubilize and extract membrane proteins from samples

Are detergents denaturing or non-denaturing with respect to protein structure?

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).

What are detergents?

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.

How does detergent-based cell lysis work?

Detergents are amphipathic molecules, meaning they contain both a nonpolar “tail” having aliphatic or aromatic character and a polar “head”. Like the components of biological membranes, detergents have hydrophobic-associating properties as a result of their nonpolar tail groups. Nevertheless, detergents are themselves water soluble.

Consequently, detergent molecules allow the dispersion (miscibility) of water-insoluble, hydrophobic compounds into aqueous media, including the extraction and solubilization of membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization.

What types of detergents are available for cell lysis?

Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking proteinprotein interaction. These detergents are considered harsh. Non-denaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS). These detergents do not denature proteins and do not break protein-protein interactions. These detergents are considered mild.

Why does the method of cell lysis matter?

Cell lysis is the first step in cell fractionation, organelle isolation, and protein extraction and purification. As such, cell lysis opens the door to a myriad of proteomics research methods. Many techniques have been developed and used to obtain the best possible yield and purity for different species of organisms, sample types (cells or tissue), and target molecule or subcellular structure. Subcellular fractionation and protein enrichment are important methods in the rapidly growing field of proteomics. Isolation of subcellular fractions and concentration of proteins in low abundance allow for more efficient identification and study of proteins of interest. Examples are the isolation of integral membrane proteins and nuclear proteins.

What methods of cell lysis are available?

Historically, physical lysis was the method of choice for cell disruption and extraction of cellular contents; however, it often requires expensive, cumbersome equipment and involves protocols that can be difficult to repeat due to variability in the apparatus (such as loose-fitting compared with tight-fitting homogenization pestles). Also, traditional physical disruption methods are not conducive for high-throughput and smaller volumes typical of modern laboratory research.
In recent years, detergent-based cell lysis methods have become the norm. Through empirical testing by trial and error, different detergent-based solutions composed of particular types and concentrations of detergents, buffers, salts and reducing agents have been developed to provide the best possible results for particular species and types of cells. Detergents have both lysing and solubilizing effects.

For Research Use Only. Not for use in diagnostic procedures.