Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin

Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 20236

IMMOB CARBOXYPEPTIDASE Y 2ML

UNSPSC: 12161502

Code: PN

Additional Details:
Additional Details: CAS Number: 12030-88-5 Weight: 0.80000kg



Product Code. 11816744

Quantity Price
1 £ 205.0 / 2mL
Estimated Shipment date
from Supplier 27-01-2017
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Description and Specification

Specification

Description Pierce Immobilized Carboxypeptidase Y
Formulation Carboxypeptidase Y on crosslinked 4% beaded agarose, slurried in 50% glycerol with sodium azide
MS-Compatible No
Quantity 2mL
Sufficient For At least 12.5 units of activity/mL resin

Carboxypeptidase Y is a glycoprotein exopeptidase of the serine class. Carboxypeptidase Y has a broad amino acid specificity, and is able to release every amino acid including proline from the carboxyl-terminus of peptides, although glycine and aspartic acid are released much more slowly than other residues. It is active in urea and SDS solutions and retains its activity under the denaturing conditions used for polypeptide sequencing. Immobilized carboxypeptidase Y can be substituted for free carboxypeptidase Y in any application, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the carboxypeptidase Y. Immobilized carboxypeptidase Y is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.

Carboxypeptidase Y (part number PI20212 only):

  • Source: Baker's yeast
  • Specific Activity: ∽100 units/mg
  • Supplied: Lyophilized in 0.1 M sodium citrate, pH 5.3

Immobilized Carboxypeptidase Y:

  • Activity: ≥5 units/mL of resin
  • Supplied: 50% glycerol, 0.02% sodium azide
  • Support: Crosslinked 4% beaded agarose
  • Spacer: Diaminodipropylamine

Documentation

Technical documents