Thermo Scientific™ Pierce™ Carboxypeptidase Y and Resin

Cleave (release) terminal amino acids including proline from the C-terminus of peptides using this protease; its immobilized form allows enzyme removal after treatment.

Brand:  Thermo Scientific™ 20236

Product Code. 11816744

Description

Thermo Scientific™ Pierce Carboxypeptidase Y is a protease with that is able to cleave (release) any terminal amino acid including proline from the C-terminus of peptides; the immobilized form allows sample separation after treatment.

Carboxypeptidase Y is a glycoprotein exopeptidase of the serine class. Carboxypeptidase Y has a broad amino acid specificity, and is able to release every amino acid including proline from the carboxyl-terminus of peptides, although glycine and aspartic acid are released much more slowly than other residues. It is active in urea and SDS solutions and retains its activity under the denaturing conditions used for polypeptide sequencing. Immobilized carboxypeptidase Y can be substituted for free carboxypeptidase Y in any application, and is advantageous because it virtually eliminates autolysis, eliminates contamination of a sample with the protease and allows control of the digestion by removing the carboxypeptidase Y. Immobilized carboxypeptidase Y is also more stable against heat-induced denaturation, resulting in longer maintenance of activity.

Carboxypeptidase Y (part number PI20212 only):

• Source: Baker's yeast
• Specific Activity: ∽100 units/mg
• Supplied: Lyophilized in 0.1 M sodium citrate, pH 5.3

Immobilized Carboxypeptidase Y:

• Activity: ≥5 units/mL of resin
• Supplied: 50% glycerol, 0.02% sodium azide
• Support: Crosslinked 4% beaded agarose
• Spacer: Diaminodipropylamine