Thermo Scientific™ Pierce™ Biotin-Conjugated Proteins and Molecules

Purified biotin and biotinylated BSA, HRP, AP or FITC for use as controls or to amplify signal in IHC via the avidin-biotin complex (ABC).

Brand: Thermo Scientific™

Manufacturer Part Number: 22030


UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 2420-94-2 Weight: 0.02000kg

Product Code. 10752905

Quantity Price
1 £287.00 / 5mg
Estimated Shipment date
from Supplier 03-11-2016
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  • Description and Specification
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Description and Specification


Description Pierce Biotin-Fluorescein Conjugate
Formulation Salt-free powder
Quantity 5mg

Biotinylated HRP and biotinylated AP are most commonly used in immunohistochemistry (IHC) to amplify the signal of biotinylated primary antibodies using the ABC staining method. Biotinylated Fluorescein (FITC) cannot be used to polymerize and amplify the signal to the same degree as biotinylated enzymes because each fluor molecule is tagged with only one biotin molecule. However, the fluorescent variant of traditional ABC staining is possible with this unique molecule.


  • Biotinylated enzymes – biotin-labeled horseradish peroxidase (B-HRP), alkaline phosphatase (B-AP) and beta-galactosidase (B-bGal) for use in avidin-biotin complex (ABC) staining

  • Biotinylated FITC – amplify the fluorescein signal of biotinylated primary antibodies by using avidin or streptavidin plus biotinylated fluorescein (B-FITC)

  • Biotinylated controls – use biotinylated bovine serum albumin (B-BSA) or any one of these molecules as positive controls in biotin quantitation methods

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