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Thermo Scientific™ IPTG

Detect beta-galactosidase expression from vectors, plasmids, or DNA fragments with this chromogenic substrate.

Brand:  Thermo Scientific™ 34060

55.00 GBP valid until 2024-03-29
Use promo code "21615" to get your promotional price.



Code : PN

Additional Details : Weight : 1.81440kg

Product Code. 10658264

  • £82.50 / 1g
Estimated Shipment: 09-04-2024
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Description

Description

Thermo Scientific™ Pierce beta-Galactosidase Precipitating Substrates is a chromogenic substrate for beta-galactosidase that is used to detect expression of vectors, plasmids, or DNA fragments that contain the reporter gene.

X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic substrate forβ-Galactosidase. It yields a blue precipitate, is soluble in dimethylformamide, and can be used to detect vectors, plasmids or DNA fragments that contain the β-Galactosidase gene.

Highlights:

  • See item-specific features

X-Gal (5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside)

  • Chromogenic substrate for β-Galactosidase
  • Yields a blue precipitate
  • Soluble in DMF
  • Can be used to detect vectors, plasmids, or DNA fragments that contain the β-Galactosidase gene

IPTG (Isopropyl-β-D-thiogalactopyranoside)

  • Maximizes the expression of genes in expression vectors
  • When screening a lambda bacteriophage library with an antibody, the probability of success can be increased by preventing the expression of the fusion protein until the plaques are well established
  • By placing a nitrocellulose filter containing the inducer IPTG onto the plate and continuing growth at 37°C, the expression of the potential β-galactosidase-cDNA fusion protein can be induced after three to four hours of plaque growth; the nitrocellulose filter is then screened with antibodies
  • IPTG is also used in combination with X-Gal to detect active β-galactosidase
  • Practical in applications using Bacteriophage M13 and Igt11 vectors
Specifications

Specifications

Store at or below -20°C.
Powder
1 g
IPTG (Isopropyl β-D-thiogalactopyranoside)
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For Research Use Only. Not for use in diagnostic procedures.