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Thermo Scientific™ Pierce™ BCA™ Protein Assay, Reducing Agent Compatible (RAC)

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

Brand:  Thermo Scientific™ 23252

Code : PN

Additional Details : Weight : 0.10000kg Transport : UN number : 3316 Chem class : 1 Pack group : III


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Product Code. 10209523

  • £190.00 / 1 set


Estimated Shipment
02-01-2018
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Description

Description

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

  • Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

  • BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

  • Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

  • High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

Specifications

Specifications

Pierce BCA Microplate Protein Assay Kit, Reducing Agent Compatible (RAC)
96-Well Microplates, 20 plates
980 microplate assays
Six-component kit with BCA reagents, standard and plates
225mL kit
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • Pierce Microplate BCA Protein Assay Kit - Reducing Agent Compatible

Signal Word
  • Danger

Hazard Category
  • Acute toxicity Category 3
  • Respiratory sensitiser Category 1
  • Skin sensitiser Category 1
  • ACUTE AQUATIC Acute 1
  • LONG-TERM AQUATIC HAZARD Chronic 2

Hazard Statement
  • H301-Toxic if swallowed.
  • H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
  • H317-May cause an allergic skin reaction.
  • H400-Very toxic to aquatic life.
  • H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P284-[In case of inadequate ventilation] wear respiratory protection.
  • P273-Avoid release to the environment.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P391-Collect spillage.
  • P302+P352-IF ON SKIN: Wash with plenty of water/A§.
  • P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ A§.
  • P301+P310-IF SWALLOWED: Immediately call a POISON CENTER/doctor / A§
  • P405-Store locked up.
  • P501-Dispose of contents/container to A§.

Supplemental information
  • MIXTURE LIST-Contains : sodium carbonate, [2, 2'-Biquinoline]-4,4'-dicarboxylic acid, sodium salt (1:2), Sulfuric acid copp

Documentation

Technical Documents

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