The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.
Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP
BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required
Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample
Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)
Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
High linearity – linear working range for BSA equals 125 to 2000μg/mL
BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates