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Thermo Scientific™ 96-Well Plates for Pierce™ BCA-RAC Assay

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Quantity:
5 plates
Unit Size:
Pack of 5
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Product Code. 10166393

Brand: Thermo Scientific™ 15045

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29.52 GBP valid until 2025-12-19
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This item is not returnable. View return policy

Description

Reducing agent-compatible version of our popular BCA Reagent Kit to measure protein concentration in samples containing DTT or BME thiol-reductants.

This reducing agent-compatible (RAC) version of the Thermo Scientific™ Pierce BCA Protein Assay provides accurate determination of protein concentration in samples that contain dithiothreitol (DTT), 2-mercaptoethanol (BME), TCEP and other disulfide reducing agents.

The BCA Protein Assay Kit - Reducing Agent Compatible (BCA-RAC) provides all of the advantages of the original BCA Assay, plus compatibility with disulfide reducing agents at concentrations routinely used in protein sample buffers. This special adaptation of the popular Pierce BCA Protein Assay method enables accurate protein concentration measurement for samples containing DTT, 2-ME or TCEP. Available in tube and microplate formats, these reducing agent compatible (RAC) BCA Kits extend the already broad reagent compatibility of the BCA Protein Assay protocol to include nearly all types of components commonly present in protein research samples.

Highlights:

  • Compatible – assay samples that contain up to 5mM DTT, 35mM BME, or 10mM TCEP

  • BCA technology – only a slight modification of the standard BCA Protein Assay protocol (15-minute incubation with Compatibility Reagent); no precipitation steps required

  • Small samples – requires only 25μL (standard kit) or less than 10μL (microplate kit) of sample

  • Colorimetric – measure with a standard spectrophotometer or plate reader (562nm)

  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods

  • High linearity – linear working range for BSA equals 125 to 2000μg/mL

Includes:

BCA Reagents A and B, Compatibility Reagent, Reconstitution Buffer, and Albumin Standard (2mg/mL); Microplate kit also includes assay-compatible 96-well microplates

TRUSTED_SUSTAINABILITY

Specifications

Content And Storage Store at room temperature.
Description Pierce 96-Well Microplates for BCA-RAC Assays
Detection Method Colorimetric
Packaging 5/Pk.
Product Line Pierce
Color Clear
Product Type Microplate
For Use With (Equipment) Spectrophotometer
Material Polystyrene
No. of Wells 96
Well Shape Round Bottom (U-well)
Quantity 5 plates
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For Research Use Only. Not for use in diagnostic procedures.

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