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Thermo Scientific™ Pierce™ BCA™ Protein Assay

Green-to-blue (A562nm), precise, detergent-compatible assay reagent to measure total protein concentration vs. protein standard.

Brand:  Thermo Scientific™ 23224

Code : PN

Additional Details : CAS Number : 47-05-7 Weight : 1.81440kg Transport : UN number : 3082 Chem class : 1 Pack group : III


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Product Code. 10475944

  • £63.00 / 25mL


EU Stock 32
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Description

Description

Used in more labs than any other detergent-compatible protein assay, Pierce BCA Reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.
 
Highlights:
  • Colorimetric – estimate visually or measure with a standard spectrophotometer or plate reader (562nm)
  • Excellent uniformity – exhibits less protein-to-protein variation than dye-binding methods
  • Compatible – unaffected by typical concentrations of most ionic and nonionic detergents
  • Moderately fast – much easier and four times faster than the classical Lowry method
  • High linearity – linear working range for BSA equals 20 to 2000µg/mL
  • Sensitive – detect down to 5µg/mL with the enhanced protocol
BCA Protein Assay Applications:
  • Studying protein:protein interactions
  • Measuring column fractions after affinity chromatography
  • Estimating percent recovery of membrane proteins from cell extracts
  • High-throughput screening of fusion proteins
Specifications

Specifications

Pierce BCA Protein Assay Reagent B
4% copper sulfate pentahydrate solution
25mL
5000 microplate assays when mixed with Reagent A
Videos
Safety and Handling

Safety and Handling

Product Identifier
  • Pierce(R) BCA Protein Assay Reagent B, 25 mL

Signal Word
  • Warning

Hazard Category
  • ACUTE AQUATIC Acute 1
  • LONG-TERM AQUATIC HAZARD Chronic 2

Hazard Statement
  • H400-Very toxic to aquatic life.
  • H411-Toxic to aquatic life with long lasting effects.

Precautionary Statement
  • P273-Avoid release to the environment.
  • P391-Collect spillage.

Supplemental information
  • MIXTURE LIST-Contains¬†: Copper (II) sulfatepentahydrate

Documentation

Technical Documents

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