missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Western Blot Products
  4. X-Ray Film and Supplies
  5. 10219853

Thermo Scientific™ Pierce™ Background Eliminator Kit

Product Code. 10219853 Shop All Thermo Scientific Products
Click to view available options
Quantity:
200 mL
Unit Size:
Each
This item is not returnable. View return policy

Product Code. 10219853

Brand: Thermo Scientific™ 21065

Please to purchase this item. Need a web account? Register with us today!

Denna artikel kan inte returneras. Se returpolicy

Beskrivning

Rapidly remove haze and artifacts from X-ray film to correct overexposed blots, whether from chemiluminescent Western blot or DNA or RNA autoradiography.

Thermo Scientific™ Pierce Background Eliminator provides fast, easy removal of haze and artifacts on developed X-ray film to correct for overexposure of blots, whether from chemiluminescent Western blotting or DNA or RNA autoradiography.

This two-reagent kit is simple to use. Mix the two reagents (1:1 ratio) and immerse a developed piece of film (recent or old) in the working solution. Watch for 1 to 5 minutes until the desired image appears, then stop the reaction by rinsing the film in water. Pierce Background Eliminator for Film evenly reverses development-signal over the entire film, preserving relative densitometry values so that analysis and publication of results is still valid and possible.

Highlights:

  • For overexposed X-ray film – reverses development of silver-based autoradiography film removing silver emulsion from the film surface

  • Recovers and preserved data – evenly reduces developed signal over the entire film so relative intensity (densitometry) of bands is not altered

  • Optimizes for signal-to-noise – allows readjustment of overdeveloped film to obtain the best image with respect to background haze, speckles and oversaturated bands that need to be resolved

  • Saves time and resources – eliminates the need to repeat experiments or re-expose a new piece of film to obtain an optimal image

Recommended for:

Remove background from any application that uses X-ray film exposures including: Western, Northern and Southern blots that use Thermo Scientific SuperSignal Substrates and the Pierce Chemiluminescent Hybridization and Detection Kit; In-gel detection systems; Gel-shift assays; Ribonuclease protection assays (RPA)

TRUSTED_SUSTAINABILITY

Specifikationer

Product Type Background Eliminator Kit
Quantity 200 mL
Detection Method Western Blot
Reactivity All species
Content And Storage Sufficient For: 2.5L of 1X solution to treat at least 10 films (13 x 18 cm ea.)
• Reagent A, 100 mL
• Reagent B, 100 mL

Store at room temperature
Product Line Pierce

For Research Use Only. Not for use in diagnostic procedures.

Korrigering av produktinnehåll

Din input är viktig för oss. Fyll i det här formuläret för att ge feedback relaterad till innehållet på denna produkt.

Produkttitel

Genom att klicka på Skicka bekräftar du att du kan bli kontaktad av Fisher Scientific angående feedbacken du har lämnat i detta formulär. Vi kommer inte att dela din information för andra ändamål. All kontaktinformation som tillhandahålls ska också underhållas i enlighet med vår Sekretesspolicy.

Tack! Din feedback har skickats. Fisher Scientific arbetar alltid för att förbättra vårt innehåll åt dig. Vi uppskattar din feedback.