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Avidin Protein and Conjugates, Invitrogen™

Perform Western blot, ELISA or other biotin-based detection methods using these purified and HRP-, AP- or FITC-labeled avidin molecules.

Brand:  Invitrogen 21221

UNSPSC : 41105300

Code : PN

Additional Details : CAS Number : 110-26-9 Weight : 0.80000kg

Product Code. 11881185

  • £181.00 / 5mg


Estimated Shipment
09-10-2017
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Description

Description

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Highlights:

  • Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10

  • Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin

  • Affordable – significantly less expensive than recombinant streptavidin

  • ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)

  • Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays

Specifications

Specifications

Pierce Avidin, Fluorescein (FITC) Conjugated
Approx. 5mg/mL in buffered saline solution
5mg
Documentation

Technical Documents