Avidin Protein and Conjugates, Invitrogen™

Perform Western blot, ELISA or other biotin-based detection methods using these purified and HRP-, AP- or FITC-labeled avidin molecules.

Overview
Brand: Invitrogen

Manufacturer Part Number: 21221

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: CAS Number: 110-26-9 Weight: 0.80000kg



Product Code. 11881185

Quantity Price
1 £ 181.0 / 5mg
Estimated Shipment date
from Supplier 07-04-2017
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Description and Specification

Specification

Formulation Approx. 5mg/mL in buffered saline solution
Description Pierce Avidin, Fluorescein (FITC) Conjugated
Quantity 5mg

Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.

Highlights:

  • Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10

  • Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin

  • Affordable – significantly less expensive than recombinant streptavidin

  • ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)

  • Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays

Documentation

Technical documents