Avidin from chicken egg whites is a glycoprotein that effectively binds biotin, a tag that is frequently used to tag antibodies and other probes for immunodetection methods. Pure avidin is a key component in certain immunohistochemistry (IHC) methods. Alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugates of avidin are useful for Western blotting or ELISA detection with appropriate enzyme substrates.
Native avidin – glycoprotein from chicken egg whites, 67kDa, pI equals 10
Soluble – glycosylation and isoelectric point renders avidin more soluble than streptavidin
Affordable – significantly less expensive than recombinant streptavidin
ABC staining – avidin is usually preferred over streptavidin for avidin-biotin complex (ABC) staining methods in immunohistochemistry (IHC)
Pure protein and conjugates – choose pure, unconjugated avidin for use in ABC staining or peroxidase or alkaline phosphatase forms for membrane and plate-based assays