Thermo Scientific™ Pierce™ Amino Acids for SILAC Labeling

Study protein expression by mass spectrometry with mass variants of lysine, arginine and other amino acids to perform stable isotope labeling with amino acids in cell culture.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 89990

UNSPSC: 41115404

Code: PN

Additional Details:
Additional Details: Weight: 0.45360kg



Product Code. 10065703

Quantity Price
1 £ 346.0 / 50mg
EU Stock 21
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Description and Specification

Specification

Formulation Lyophilized powder of L-Arginine-HCl with at least 98% 13C6, 15N4
Sufficient For Media for one SILAC experiment
Description L-Arginine-HCl, 13C6, 15N4
Quantity 50mg
For Use With (Application) SILAC experiments (stable isotope labeling with amino acids in cell culture)

SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of “heavy“ 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization and quantitation of proteins.

Highlights:

  • Efficient – 100% label incorporation into proteins of living cells

  • Flexible – different isotopes of heavy and/or light amino acids for arginine, lysine, leucine and proline enable the quantitation of peptides derived from MS-grade proteases

  • Multiplex capabilities – several alternative isotopes of arginine and lysine are available that allow the analysis of multiple treatment conditions in each experiment

  • High-quality supplements – heavy amino acids with >99% isotope purity

Documentation

Technical documents