Invitrogen™ AKT Multispecies In-Cell ELISA Kit, Near Infrared

Product Code.	Quantity	unitSize
11568100	96 Tests	Each

Product Code. 11568100 Supplier Invitrogen™ Supplier No. 62220

Description

In-cell ELISA Kit

The AKT Multispecies In-Cell ELISA Near Infrared Detection Kits provide a simple and convenient method for quantifying intracellular proteins in whole cells. Principle of the method This kit enables simultaneous detection of post-translational-modified protein (PTM) and the corresponding unmodified protein, or two different targets within the same well. Simultaneously detecting targets within an ELISA well eliminates variability caused by differences in cell plating. The expression levels of the protein(s) are monitored using primary antibodies specific to the targets and corresponding species-specific near-infrared Thermo Scientific DyLight-Conjugated Secondary Antibodies. Relative protein activation or inactivation is determined as a ratio of modified protein levels to the corresponding unmodified protein measured using a modification-specific antibody and an antibody to unmodified protein, respectively. Rigorous validation Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.

AKT (protein kinase B, PKB, RAS-alpha) is a 57kDa serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. AKT is activated by insulin, PI3K, IGF1 and various other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82% identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH) domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. The AKT pathway is a major target for cancer therpeutics where AKT signaling dysfunction has implicated in many types of cancer, including cancer syndromes known as phakomatoses.

Specifications

• Accession Number: P31749, P31751, Q9Y243
• Description: Pierce AKT Near Infrared In-Cell ELISA Kit
• Conjugate: Unconjugated
• Immunoassay Kit Format: Multi-component kit with fluorescent conjugates
• Product Type: ELISA Kit
• Sample Type: Supernatant
• Sufficient For: 96 microplate wells of overnight-cultured cells
• Target Species: Human
• For Use With (Equipment): Fluorescent Microplate Reader
• Gene ID (Entrez): 10000,207,208
• Gene Symbol: AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-PK-gamma,RAC-gamma,STK-2
• Kit Contents: Blocking Buffer, 20X Tris Buffered Saline, Surfact-Amps 20, Surfact-Amps X-100, DyLight™ 680 Goat Anti-Mouse IgG (H+L), DyLight 800 Goat Anti-Rabbit IgG (H+L), Thin Plate Seal Assembly, Components included only in the target-specific kits:Antibody #1 and Antibody #2
• Label or Dye: DyLight™ 680, DyLight™ 800
• Quantity: 96 Tests
• Regulatory Status: RUO
• Sample Volume: Supernatant, 10,000 cells
• Storage Requirements: 2°C to 8°C
• Target: Human
• Test Time: 1 hr. 20 min.
• Total Assay Time: 24 hr.