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Thermo Scientific™ Pierce™ ABTS Substrates

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Brand:  Thermo Scientific™ 37615

UNSPSC : 41105300

Code : PN

Additional Details : Weight : 0.25000kg

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Product Code. 10269423

  • £88.00 / 250mL


EU Stock 13
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Description

Description

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights:

  • Excellent choice when maximum sensitivities are not required
  • Less readily oxidized and color development is slower (approximately 20 minutes) than OPD and TMB
Specifications

Specifications

Pierce 1-Step ABTS Solution
Proprietary buffered solution containing 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt
250mL
At least 15 x 96-well microplates using 150uL per well