Thermo Scientific™ Pierce™ ABTS Substrates

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 37615

250 ML ABTS SUBSTRATE, 1-STEP 250ML STORE INFridge at 4C

UNSPSC: 41105300

Code: PN

Additional Details:
Additional Details: Weight: 0.25000kg



Product Code. 10269423

Quantity Price
1 £ 88.0 / 250mL
EU Stock 25
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Description and Specification

Specification

Description Pierce 1-Step ABTS Solution
Formulation Proprietary buffered solution containing 2,2'-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt
Quantity 250mL
Sufficient For At least 15 x 96-well microplates using 150uL per well

ABTS (2,2'-Azinobis [3-ethylbenzothiazoline-6-sulfonic acid]-diammonium salt) is a water-soluble HRP substrate that yields a green end product upon reaction with peroxidase. The green product has two major absorbance peaks, 410nm and 650nm. ABTS is less sensitive than OPD and TMB in ELISA applications. It is less readily oxidized, and its color development is slower (approximately 20 minutes). This may be advantageous if unacceptable background results from the use of the OPD or TMB substrates due to higher sensitivities.

Highlights:

  • Excellent choice when maximum sensitivities are not required
  • Less readily oxidized and color development is slower (approximately 20 minutes) than OPD and TMB