Thermo Scientific™ Pierce™ 6xHis Protein Tag Staining Kit

Preferentially detect histidine-tagged (His-tagged) recombinant fusion proteins in polyacrylamide gels with this fluorescent stain.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: 24570

UNSPSC: 41105322

Code: PN

Additional Details:
Additional Details: Weight: 0.10000kg Transport: UN number : 1544 Chem class : 1 Pack group : III



Product Code. 10322234

Quantity Price
1 £ 272.0 / 1 set
Estimated Shipment date
from Supplier 30-03-2017
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Description and Specification

Specification

Quantity 1L Reagent Set
Formulation Two bottles of pre-formulated reagents (proprietary) (1L total)
Kit Contents 6xHis Tag Developer, 500mL
Sufficient For 10 mini gels (SDS-PAGE)
Description Pierce 6xHis Protein Tag Stain Reagent Set

The unique fluorescent stain specifically detects histidine-tagged fusion proteins that have been electrophoresed in polyacrylamide gels (e.g., SDS-PAGE). With this stain, expression of His-tagged recombinant proteins can be assessed directly in-gel, eliminating membrane transfer and Western blotting steps that are typically used to confirm purity and yield of tagged proteins. Although the lower limit of detection depends on which UV lamp (300nm) and detection equipment (CCD camera best) are used, the kit is convenient for in-process assessment of His-tagged protein production and is compatible with subsequent staining with coomassie dye and other total protein stains.

Highlights:

  • Two- to three-times faster than Western blotting – get results sooner and save hours of valuable research time

  • Detects directly on the gel – can eliminate the need for a membrane transfer and Western blot step for the detection of at least 0.2μg of 6xHis-tagged protein

  • Ready-to-use, two-reagent formula – no mixing, no diluting and no fuss; guarantees a simple-to-perform, mistake-free detection protocol

  • Specific fluorescent detection of 6xHis-tagged proteins – see only what you want to see (CCD camera recommended for low abundance proteins; UV-transilluminator for abundant proteins)

  • Compatible with double-staining – follow His-tagged protein staining with Thermo Scientific GelCode Blue Stain Reagent for a total protein profile determination

Documentation

Technical documents