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Invitrogen™ Phycoerythrin Monoclonal Antibody (eBioPE-DLF), eBioscience™, Invitrogen™

Description
Description: The eBioPE-DLF monoclonal antibody reacts with phycoerythrin (PE), PE-Cy5 and PE-Cy7, with no crossreactivity to fluorescein (FITC) or allophycocyanin (APC). When using eBioPE-DLF as a second-step antibody, in combination with an appropriate third step, to bind a PE-conjugated primary antibody, there is no decrease in the fluorescent intensity of the directly conjugated PE antibody. Therefore no interference or quenching of the PE fluorochrome is observed. The eBioPE-DLF can be used for separation of cells labeled with PE-conjugated antibodies or staining. Please note that the optimal concentration for this antibody will vary depending on the density of the primary PE-conjugated antibody and the application tested. Applications Reported: This eBioPE-DLF antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioPE-DLF antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 μm post-manufacturing filtered.
Specifications
Specifications
| Antigen | Phycoerythrin |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | eBioPE-DLF |
| Concentration | 0.5 mg/mL |
| Conjugate | Unconjugated |
| Formulation | PBS with 0.09% sodium azide; pH 7.2 |
| Gene Alias | PE; RPE; R-PE; R-phycoerythrin |
| Host Species | Mouse |
| Purification Method | Affinity chromatography |
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For Research Use Only.
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