Thermo Scientific™ Phusion™ Hot Start II High-Fidelity DNA Polymerase

Optimize PCR with one of the most accurate hot start DNA polymerases on the market, featuring extreme specificity and improved performance.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: F-549S

X100 UNITS PHUSION HOT START II HIGH-FIDELITY DNA

UNSPSC: 41106300

Code: A0

Additional Details:
Additional Details: Weight: 0.02000kg



Disclaimers: Current Phusion Hot Start customers can switch to Phusion Hot Start II with no changes needed to existing protocols or primers. Original Phusion Hot Start will remain available for purchase, however, all new customers are advised to purchase Phusion Hot Start II to allow more flexibility in primer design.

Product Code. 10441338

Quantity Price
1 £ 94.0 / 100 units
EU Stock 123
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Description and Specification

Specification

Description Phusion™ Hot Start II High-Fidelity DNA Polymerase
For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration
Quantity 100U (2U/µL)
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.

Phusion Hot Start High-Fidelity DNA Polymerase is a highly accurate hot start DNA polymerase. It has now been upgraded to allow the use of even wider range of primers, including those with low melting temperatures. The new Phusion Hot Start II DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications such as cloning and high-throughput PCR.
  • No annealing temperature limitations
  • Allows reaction setup at room temperature
  • Zero-time reactivation due to unique hot start technology
  • Fast PCR enzyme with high fidelity (over 6 times more accurate than Pfu)
  • High product yields with low enzyme amounts gives excellent cost/reaction ratio
  • Robust enzyme with high tolerance against PCR inhibitors requires minimal optimization

Includes:

5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

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