missing translation for 'onlineSavingsMsg'
Learn More

Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Brand:  Thermo Scientific™ F532S

UNSPSC : 41106300

Code : 87

Additional Details : Weight : 1.51950kg

 View more versions of this product

Product Code. 10523288

  • £134.00 / 100 reactions


In Stock 1
EU Stock 9
Add to basket

Disclaimers:

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.


Specifications

Description

Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user.
  • High fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Speed of the polymerase allows short extension times (15-30 s/kb)
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts

Includes:

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Description & Specifications

Specifications

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
With GC Buffer
100 x 50uL Rxns
Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.
Documentation

Technical Documents

Alternative Products
Special Offers

Special Offers

  • Thermo Scientific
    Since their introduction in 2003, Thermo Scientific Phusion High-Fidelity DNA Polymerases have established a new standard for high-fidelity PCR.