Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: F-532S

100 RXN PHUSION HIGH-FIDELITY PCR MASTER MIX GCbuffer formulation (2 x 1.25mL), 2X Master Mix

UNSPSC: 41106300

Code: 87

Additional Details:
Additional Details: Weight: 1.51950kg



Disclaimers: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Product Code. 10523288

Quantity Price
1 £ 134.0 / 100 reactions
EU Stock 16
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  • Description and Specification
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Description and Specification

Specification

For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes With GC Buffer
Quantity 100 x 50uL Rxns
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.

Phusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user.
  • High fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Speed of the polymerase allows short extension times (15-30 s/kb)
  • Robust reactions, minimal optimization needed
  • Increased product yields with minimal enzyme amounts

Includes:

2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.

Recommended for:

Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray

Alternative Products
Documentation

Technical documents

Special Offers