Thermo Scientific™ Phusion High-Fidelity DNA Polymerase
Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.
Brand: Thermo Scientific™ F531L
Code : 87
Additional Details : Weight : 0.06000kg
DescriptionPhusion High-Fidelity PCR Master Mix with HF Buffer and Phusion High-Fidelity PCR Master Mix with GC Buffer are convenient 2x mixes containing Phusion DNA Polymerase, nucleotides and optimized HF or GC reaction buffer including MgCl2. Only template and primers need to be added by the user.
- High fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
- Speed of the polymerase allows short extension times (15-30 s/kb)
- Robust reactions, minimal optimization needed
- Increased product yields with minimal enzyme amounts
2x Phusion High-Fidelity PCR Master Mix with HF Buffer contains Phusion DNA Polymerase, 2x Phusion HF Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product. 2x Phusion High-Fidelity PCR Master Mix with GC Buffer contains Phusion DNA Polymerase, 2x Phusion GC Buffer and 400μM of each dNTP. The master mix supplies 1.5 mM MgCl2 and 200μM dNTP in final reaction concentration. A separate tube of DMSO is provided with the product.
Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
|Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray|
|With High-Fidelity buffer|
|500 x 50uL Rxns|
|Recommended storage temperature -20°C. Stable for one year from the packaging date. After thawing the mix can be stored at +4°C for three months.|
Since their introduction in 2003, Thermo Scientific Phusion High-Fidelity DNA Polymerases have established a new standard for high-fidelity PCR.
Save up to 50% on related products from the Thermo Scientific cloning lineup: 12936
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.