Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Brand: Thermo Scientific™

Manufacturer Part Number: F-530L

1 SET PHUSION HIGH-FIDELITY DNA POLYMERASE 500 U,supplied with 5x Phusion HF buffer, 5x Phusion GC

UNSPSC: 41106300

Code: 87

Additional Details:
Additional Details: Weight: 0.02100kg

Disclaimers: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Product Code. 10287787

Quantity Price
1 £350.00 / 500 units
EU Stock 61
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Description and Specification


For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration.
Quantity 500U (2U/µL)
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the assay date.


  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Green Buffer


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