Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

Brand: Thermo Scientific™

Manufacturer Part Number: F-553S

50 RXN PHUSION HIGH-FIDELITY PCR KIT, 50 U, WITHboth 5x Phusion HF buffer (1.5mL) and 5x Phusion

UNSPSC: 41106300

Code: 87

Additional Details:
Additional Details: Weight: 0.05000kg

Disclaimers: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser≈s own internal research. No other patent rights are conveyed expressly, by implication, or by estoppels. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Product Code. 10044257

Quantity Price
1 £53.50 / 50 reactions
EU Stock 14
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Description and Specification


For Use With (Application) Fast PCR, high-fidelity PCR, cloning, long or difficult amplicons, DHPLC, microarray
Includes Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS
Quantity 50 x 50µL Rxns (125 x 20µL Rxns)
Storage Requirements Recommended storage temperature -20°C. Stable for one year from the packaging date.


  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Green Buffer