Thermo Scientific™ Phusion™ Blood Direct PCR Kit

Perfrom PCR directly from whole blood without DNA purification.

Overview
Brand: Thermo Scientific™

Manufacturer Part Number: F-547L

500 RXN PHUSION BLOOD DIRECT PCR KIT, PHUSIONBlood II DNA Polymerase (200µL), 2x PhusionBlood

UNSPSC: 41106615

Code: 87

Additional Details:
Additional Details: Weight: 1.71460kg



Product Code. 10227847

Quantity Price
1 £ 346.0 / 500 reactions
EU Stock 9
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Description and Specification

Specification

For Use With (Application) amplification of DNA directly from whole blood.
Includes Phusion Blood DNA Polymerase, 2x Phusion Blood PCR Buffer (includes dNTPs and MgCl2), EDTA, MgCl2, DMSO, universal control primer mix
Product Type Phusion™ Blood Direct PCR Kit
Quantity 500 reactions
Storage Requirements Store at -20°C.

Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood without a prior purification step. The kit utilizes a modified Phusion Hot Start II High-Fidelity DNA Polymerase. This highly processive and robust PCR enzyme is resistant to the inhibitors present in blood and retains its activity even at 40% blood concentration. Phusion Blood Direct PCR Kit allows analysis of blood samples with different anticoagulants, storage conditions and from various species.
  • No need for DNA extraction
  • Allows up to 40% blood in the PCR reaction
  • Tolerates heparin, citrate, and EDTA, and is compatible with frozen blood or blood stored on various types of filter papers
  • Modified Phusion Hot Start II DNA Polymerase gives specific and accurate results with high yields and short protocol times

Includes:

Phusion Blood DNA Polymerase, 2x Phusion Blood PCR Buffer (includes dNTPs and MgCl2), EDTA, MgCl2, DMSO, universal control primer mix

Recommended for:

Detection of SNPs with PCR-RFLP. Genotyping.

Documentation

Technical documents