Phosphotyrosine Monoclonal Antibody (M375), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA547634

Product Code. 17836818

Description

This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine -phosphorylated proteins and the biochemical pathways in which they function. The fluorescein (FITC) conjugate of clone PY20 anti-phosphotyrosine is especially useful for the detection of these P-Tyr proteins in immunohistochemical and immunocytochemical protocols in situations wherein the use of a secondary antibody would complicate detection of the protein(s) of interest.

Specifications

• Antigen: Phosphotyrosine
• Classification: Monoclonal
• Concentration: Lot-Specific
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P29474, P70313, Q62600
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 18127, 24600, 4846
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Isotype: IgG2b

• Applications: Immunocytochemistry, Immunoprecipitation, Western Blot
• Clone: M375
• Conjugate: Unconjugated
• Gene: NOS3
• Gene Alias: Phosphotyrosine; pTyr; pY
• Immunogen: Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid