Phospho-Tyrosine Antibody (PY20), DyLight 550, Novus Biologicals™

Mouse Monoclonal Antibody

Brand:  Novus Biologicals NBP2-33166R

Product Code. 18123463

Description

Phospho-Tyrosine Monoclonal specifically detects Phospho-Tyrosine in All Species samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Phospho-Tyrosine
• Classification: Monoclonal
• Conjugate: DyLight 550
• Gene Alias: 2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, phosphotyrosine, pTyrosine, Tyrosine
• Immunogen: Phosphotyrosine was used as the immunogen for the Phosphotyrosine PY20 antibody.
• Quantity: 0.1 mL
• Primary or Secondary: Primary
• Target Species: All species
• Form: Purified

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
• Clone: PY20
• Dilution: Western Blot 1:100-1:2000, Flow Cytometry 1:10-1:1000, ELISA 1-5 μg/mL for coating, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Test Specificity: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This monoclonal antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
• Content And Storage: Store at 4C in the dark.
• Isotype: IgG2b

For Research Use Only