Phospho-TRPM7 (Ser1513) Polyclonal Antibody, Invitrogen™

Rabbit Polyclonal Antibody

Brand:  Invitrogen PA5143779

Product Code. 17836858

Description

The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1513) peptide then affinity purified using phospho-TRPM7 (Ser-1513) peptide. This antibody detects a 220 kDa* protein on SDS-PAGE immunoblots of human MDA-MB-231 and rat PC12 cells stimulated with calyculin A, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

Specifications

• Antigen: Phospho-TRPM7 (Ser1513)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Trpm7
• Gene Alias: 2310022G15Rik; 4833414K03Rik; 5033407O22Rik; ALSPDC; CHAK; CHAK1; Channel kinase 1; Channel-kinase 1; FLJ20117; long transient receptor potential channel 7; long transient receptor potential channel 7 {ECO:0000250; Ltpr7; LTRPC ion channel family member 7; LTRPC7; LTrpC-7; LTrpC7 {ECO:0000250; PubMed:11161216}; RGD:620053}; tr; transient receptor potential cation channel subfamily M member 7; transient receptor potential cation channel subfamily M member 7 {ECO:0000312; transient receptor potential cation channel, subfamily M, member 7; transient receptor potential M7; transient receptor potential-phospholipase C-interacting kinase; transient receptor potential-phospholipase C-interacting kinase {ECO:0000303; transient receptor potential-related protein, ChaK; TRMP7; TRP PLIK; Trpm7; trpm7 {ECO:0000312; TRPPLIK; Trp-plik; TRP-PLIK {ECO:0000303; UniProtKB:Q96QT4}
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 54822, 58800, 679906
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Isotype: IgG

• Applications: Western Blot
• Concentration: Lot-Specific
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: Q923J1, Q925B3, Q96QT4
• Gene Symbols: Trpm7
• Immunogen: Phospho-TRPM7 (Ser-1513) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1513 in human TRPM7. This site is well conserved in rat and mouse TRPM7, but has low homology to other TRPM family members.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid