Phospho-STAT4 (Y693) Mouse anti-Human, Mouse, APC, Clone: 4LURPIE, eBioscience™

Mouse Monoclonal Antibody

Overview
Brand: Affymetrix eBioscience

Manufacturer Part Number: 17-9044-42

100TEST Anti-Human/Mouse phospho-STAT4 (Y693) APC

Code: NEW

Additional Details:
Additional Details: Weight: 0.23750kg



Disclaimers: For Research Use Only.

Product Code. 15598226

Quantity Price
1 £ 287.9 / 100 tests
Estimated Shipment date
from Supplier 20-01-2017
Add to basket


Description and Specification

Specification

Antigen Phospho-STAT4 (Y693)
Applications Flow Cytometry (Intracellular Staining)
Clone 4LURPIE
Concentration 5μL (0.25μg)/test
Conjugate APC
Format Conjugated
Formulation aqueous buffer, 0.09% sodium azide, may contain carrier protein/stabilizer
Gene Alias Signal transducer and activator of transcription 4
Host Species Mouse
Isotype IgG1
Quantity 100 tests
Regulatory Status RUO
Species Reactivity Human, Mouse
Storage Requirements Store at 2-8°C. Do not freeze. Light-sensitive material.
Primary or Secondary Primary
Monoclonal or Polyclonal Monoclonal

This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693). STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members. Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner. STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta). STAT4 is required for optimal Th1 differentiation in vivo. Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient. Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice. In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells.

Specificity of this 4LURPIE clone was determined by ELISA and flow cytometry.