Invitrogen™ Phospho-RIP1 (Ser166) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5121351

Product Code. 17792744

Description

Positive test controls include: HT-29, NIH/3T3. Immunogen sequence: MWSKL.

Serine-threonine kinase which transduces inflammatory and cell-death signals (programmed necrosis) following death receptors ligation, activation of pathogen recognition receptors (PRRs), and DNA damage. Upon activation of TNFR1 by the TNF-alpha family cytokines, TRADD and TRAF2 are recruited to the receptor. Phosphorylates DAB2IP at 'Ser-728' in a TNF-alpha-dependent manner, and thereby activates the MAP3K5-JNK apoptotic cascade. Ubiquitination by TRAF2 via 'Lys-63'-link chains acts as a critical enhancer of communication with downstream signal transducers in the mitogen-activated protein kinase pathway and the NF-kappa-B pathway, which in turn mediate downstream events including the activation of genes encoding inflammatory molecules. Polyubiquitinated protein binds to IKBKG/NEMO, the regulatory subunit of the IKK complex, a critical event for NF-kappa-B activation. Interaction with other cellular RHIM-containing adapters initiates gene activation and cell death. RIPK1 and RIPK3 association, in particular, forms a necrosis-inducing complex.

Specifications

• Antigen: Phospho-RIP1 (Ser166)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: RIPK1
• Gene Alias: cell death protein RIP; D330015H01Rik; FLJ39204; OTTHUMP00000015955; receptor (TNFRSF)-interacting serine-threonine kinase 1; receptor interacting serine/threonine kinase 1; receptor-interacting protein 1; receptor-interacting protein kinase 1; receptor-interacting serine/threonine-protein kinase 1; Rinp; RIP; RIP1; RIP-1; RIP1 (CT); RIPK1; serine/threonine-protein kinase RIP
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 19766, 8737
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Western Blot
• Concentration: 2.07 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.3
• Gene Accession No.: Q13546, Q60855
• Gene Symbols: RIPK1
• Immunogen: A synthetic phosphorylated peptide around S166 of human RIPK1/RIP (NP_003795.2).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG