Phospho-PYK2 (Tyr402) Monoclonal Antibody (M269), Invitrogen™

Mouse Monoclonal Antibody

Brand:  Invitrogen MA547635

Product Code. 17836738

Description

The antibody detects a 116 kDa* band corresponding to PYK2 on SDS-PAGE immunoblots of human A431, Jurkat and K562 cells treated with pervanadate, but is not observed in control, untreated cells.

PTK2B (FAK2) is a cytoplasmic tyrosine kinase involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. PTK2B regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading, and bone remodeling. FAK2 plays a role in the regulation of the humoral immune response and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. It functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors, cytokine, chemokine, and growth factor receptors, and mediates responses to cellular stress. Aberrant FAK2 expression may play a role in cancer cell proliferation, migration, and invasion in tumor formation and metastasis. Elevated expression has been seen in gliomas, hepatocellular carcinoma, lung cancer, and breast cancer.

Specifications

• Antigen: Phospho-PYK2 (Tyr402)
• Classification: Monoclonal
• Concentration: Lot-Specific
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P70600, Q14289, Q9QVP9
• Gene Symbols: PTK2B
• Immunogen: Clone M269 was generated from a phosphopeptide containing amino acids surrounding tyrosine 402 in human PYK2. This sequence is conserved in rat and mouse PYK2, and has significant homology to the conserved site in FAK.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Form: Liquid

• Applications: Western Blot
• Clone: M269
• Conjugate: Unconjugated
• Gene: PTK2B
• Gene Alias: CADTK; CAK beta; CAKB; CAKbeta; CAK-beta; calcium-dependent tyrosine kinase; Calcium-regulated non-receptor proline-rich tyrosine kinase; cell adhesion kinase beta; cellular adhesion kinase beta; E430023O05Rik; FADK; FADK 2; FADK2; FAK; FAK1; FAK2; focal adhesion kinase 2; FRNK; PKB; pp125FAK; proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; protein-tyrosine kinase 2-beta; PTK; PTK2 protein tyrosine kinase 2 beta; PTK2B; PTK2B protein tyrosine kinase 2 beta; PYK2; RAFTK; related adhesion focal tyrosine kinase
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 19229, 2185, 50646
• Content And Storage: -20° C, Avoid Freeze/Thaw Cycles
• Isotype: IgG1