Invitrogen™ Phospho-NLRC4 (Ser533) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5143728

Product Code. 17836848

Description

The antibody was cross-adsorbed to unphosphorylated NLRC4 (Ser-533) peptide before affinity purification using phospho-NLRC4 (Ser-533) peptide. This antibody antibody detects a 110 kDa* protein on SDS-PAGE immunoblots of PMA-differentiated THP1 human macrophages. In addition, the antibody detects a mouse recombinant phospho-NLRC4 (Ser-533) peptide, but does not detect this peptide after lambda phosphatase treatment.

NLRC4 encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: Phospho-NLRC4 (Ser533)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Nlrc4
• Gene Alias: 9530011P19Rik; AIFEC; CARD, LRR, and NACHT-containing protein; Card12; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CLAN; Clan protein; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; ice protease-activating factor; ICE-protease activating factor; ipaf; NLR family CARD domain containing 4; NLR family CARD domain-containing protein 4; NLR family, CARD domain containing 4; NLRC4; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4; UNQ6189/PRO20215
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 268973, 298784, 58484
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 0.5 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: F1M649, Q3UP24, Q9NPP4
• Gene Symbols: Nlrc4
• Immunogen: Phospho-NLRC4 (Ser-533) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding serine 533 in mouse NLRC4. This sequence has high homology to human and rat NLRC4, and is not homologous to sequences in other NLR family members.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG