Invitrogen™ Phospho-NCF4 (Tyr243) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5105653

Product Code. 16672095

Description

Antibody detects endogenous levels of p40 phox only when phosphorylated at Tyr243.

The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Specifications

• Antigen: Phospho-NCF4 (Tyr243)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Ncf4
• Gene Alias: AI451400; CGD3; CTA-833B7.1; MGC3810; NCF; Ncf4; NCF-4; Neutrophil cytosol factor 4; neutrophil cytosolic factor 4; neutrophil cytosolic factor 4, 40kDa; neutrophil NADPH oxidase factor 4; OTTHUMP00000028736; OTTHUMP00000043756; P40PHOX; p40-phox; SH3 and PX domain-containing protein 4; SH3PXD4
• Host Species: Rabbit
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 17972, 4689
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 50% glycerol and 0.02% sodium azide; pH 7.4
• Gene Accession No.: P97369, Q15080
• Gene Symbols: Ncf4
• Immunogen: A synthesized peptide derived from human NCF4(Accession Q15080), corresponding to amino acid residues around phosphorylated Tyr243.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG