Invitrogen™ Phospho-MYL12A (Ser19) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5143727

Product Code. 17866948

Description

This antibody was cross-adsorbed to MLC (a.a. 11-22) then affinity purified using phospho-MLC (Ser-19) peptide (without carrier). The antibody detects a 20 kDa* band corresponding to the molecular weight of myosin light chain in human A431 treated with EGF and in mouse C2C12 treated with Calyculin A. This reactivity is not observed after lambda phosphatase treatment of MLC.

This gene encodes a nonsarcomeric myosin regulatory light chain. This protein is activated by phosphorylation and regulates smooth muscle and non-muscle cell contraction. This protein may also be involved in DNA damage repair by sequestering the transcriptional regulator apoptosis-antagonizing transcription factor (AATF)/Che-1 which functions as a repressor of p53-driven apoptosis. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 8.

Specifications

• Antigen: Phospho-MYL12A (Ser19)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Myl12a
• Gene Alias: 2900073G15Rik; AI838292; brain specific myosin regulatory light chain; C80200; Epididymis secretory protein Li 24; HEL-S-24; MLC-2; MLC20; MLC-2A; MLC-2B; MLCB; MRCL3; MRLC2; MRLC3; MYL12A; MYL12B; MYL2B; MYLC2B; myosin light chain 12A; myosin light chain, regulatory B-like; myosin regulatory light chain 12A; Myosin regulatory light chain 12B; Myosin regulatory light chain 2, nonsarcomeric; Myosin regulatory light chain 20 kDa; myosin regulatory light chain 2-A, smooth muscle isoform; Myosin regulatory light chain 2-B, smooth muscle isoform; myosin regulatory light chain 3; Myosin regulatory light chain MRLC2; Myosin regulatory light chain MRLC3; myosin regulatory light chain RLC-A; Myosin RLC; myosin RLC-A; myosin, light chain 12A, regulatory, non-sarcomeric; myosin, light polypeptide, regulatory, non-sarcomeric (20kD); NMDA receptor-interacting protein; RGD1309537; RLC; Rlc-a; SHUJUN-1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 10627, 501203, 67268
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot, Immunocytochemistry
• Concentration: 0.5 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: P13832, P19105
• Gene Symbols: Myl12a
• Immunogen: Phospho-MLC (Ser-19) synthetic peptide (coupled to KLH) corresponds to amino acid residues surrounding Ser-19 in human myosin regulatory light chain 12A (Myl12A). This peptide sequence is highly conserved in human, rat, mouse, chicken, and zebrafish Myl12A, and in other smooth muscle and non-muscle MLCs.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG