Invitrogen™ Phospho-MSK1 (Thr581) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA517858

Product Code. 11577100

Description

It is not recommended to aliquot this antibody. This antibody is not cross-reactive with MSK1 phosphorylated at other sites, nor does it detect phosphorylated RSK1, RSK2 or RSK3.

RPS6KA5 (MSK1) is a serine/threonine protein kinase that is essential for the regulation of various cellular processes. RPS6KA5 is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes. Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin. Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression. In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress. In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential.

Specifications

• Antigen: Phospho-MSK1 (Thr581)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Rps6ka5
• Gene Alias: 3110005L17Rik; 6330404E13Rik; 90 kDa ribosomal protein S6 kinase 5; AI854034; kinase MSK1; KS6A5; mitogen and stress-activated protein kinase 1; Msk1; MSPK1; Nuclear mitogen- and stress-activated protein kinase 1; ribosomal protein S6 kinase A5; ribosomal protein S6 kinase alpha-5; ribosomal protein S6 kinase, 90kDa, polypeptide 5; ribosomal protein S6 kinase, polypeptide 5; RLPK; RLSK; Rps6ka5; RSKL; RSK-like protein kinase; S6K-alpha-5
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 73086, 9252
• Content And Storage: -20°C
• Form: Liquid

• Applications: Immunoprecipitation, Western Blot
• Concentration: 28 μg/mL
• Formulation: 0.01M HEPES with 100μg/mL BSA, 50% glycerol, 0.15M NaCl and no preservative; pH 7.5
• Gene Accession No.: O75582, Q8C050
• Gene Symbols: Rps6ka5
• Immunogen: Synthetic phosphopeptide corresponding to residues surrounding pThr581 of human MSK1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG