Invitrogen™ Phospho-LIMK1/LIMK2 (Ser323, Ser314) Polyclonal Antibody

Rabbit Polyclonal Antibody

Brand:  Invitrogen™ PA5143714

Product Code. 17806838

Description

This antibody was cross-adsorbed to unphosphorylated LIMK1 (Ser-323) peptide then affinity purified using LIMK1 (Ser-323) peptide (without carrier). The antibody detects a protein with the same mobility as anti-LIMK1 (C-terminus, LP1831) in human A431 cells treated with calyculin A. This band is weak in control cells and is not detected after lambda phosphatase treatment.

Proteins containing LIM motifs are typically involved in cell fate determination and growth control. A family of proteins designated LIM kinases, including LIMK-1 and LIMK-2, has been identified. LIMK-1 has been shown to regulate the stabilization of F-Actin structures and cofilin activity, indicating that LIMK-1 plays a role in a signaling pathway involved in the regulation of cell motility and morphogenesis. LIMK-1 inhibits neuronal differentiation of PC12 cells, and is thought to act by interfering with events downstream of MAPK activation. Expression patterns of LIMK-1 and LIMK-2 suggest that these proteins may have different functions during development. A truncated form of LIMK-2 has been identified in adult testis that is thought to arise from an alternative initiation exon.

Specifications

• Antigen: Phospho-LIMK1/LIMK2 (Ser323, Ser314)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: LIMK1
• Gene Alias: KIZ-1; LIM domain kinase 1; LIM domain kinase 2; lim kinase 1; LIM kinase 2; LIM motif-containing protein kinase; LIM motif-containing protein kinase 1; LIM motif-containing protein kinase 2; LIM-domain containing protein kinase 1; LIM-domain containing, protein kinase; LIM-domain containing, protein kinase 1; Limk; LIMK 2; LIMK1; LIMK-1; LIMK2; LIMK-2; Limk2b; Link2
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 16885, 16886, 29524, 3984, 3985, 65172
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene Accession No.: O54785, P53667, P53668, P53669, P53670, P53671
• Gene Symbols: LIMK1, LIMK2
• Immunogen: LIMK1 (Ser-323) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 323 in human LIMK1. This sequence is conserved in rat and mouse LIMK1, and has high homology to Ser-314 in human LIMK2.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG